Research Paper Volume 11, Issue 7 pp 2003—2019

Figure 4. Genistein opposes P31-43 induced inflammation in intestinal epithelial cells by targeting CFTR. (A-B) Caco-2 cells incubated with or without P31-43 in the presence or absence of genistein. Protein levels (by specific ELISA) of IL-1 β (A) and IL-15 (B). Means±SD of pooled samples assayed in triplicates; ***p<0.001 untreated cells versus P31-43, °°p<0.01 P31-43 versus genistein+P31-43 (ANOVA, Bonferroni post-hoc test). (C) Immunoblot of phospho-ERK 1/2 in CFTR-WT Caco-2 cells or in CFTR-knock out Caco-2 cells treated or not with genistein (left) and densitometric analysis of protein levels relative to β-actin (right) (n=3 independent experiments). Means±SD of triplicates of independent experiments; **p<0.01 versus CFTR-knockout Caco-2 cells (ANOVA, Bonferroni post-hoc test). (D) CFTR-knockout Caco-2 cells incubated with P31-43 in the presence or absence of genistein. Protein levels (by specific ELISA) of IL-15. Means±SD of pooled samples assayed in triplicates. Data information: The blots are representative of one experiment for group of treatment.