Research Paper Volume 11, Issue 8 pp 2217—2240

Figure 5. RSV inhibits PA-induced muscle atrophy in L6 myotubes. (A) Myotubes were treated with different doses (0.25, 0.5, 0.75, or 1 mM) of PA for 24 h, and cell viability was measured using a CCK-8 assay. (B) Representative image of the western blotting results for MHC; GAPDH was used as a loading control. (C) The bar chart shows the quantification of MHC. (D) Myotubes were treated with RSV at a series of concentrations (1, 5, 10, 25 or 50 μM) for 24 h, and cell viability was detected by CCK-8 assay. (E) Myotubes were treated with 0.75 mM PA in the presence or absence of different concentrations (1, 5, 10 and 25 μM) of RSV for 24 h. The protein expression of MHC was measured by western blotting. (F) The bar graph shows the quantification of MHC. (G) Myotubes were exposed to 0.75 mM PA in the presence or absence of 25 μM RSV for 24 h, and cell viability was detected by a CCK-8 assay. (H) Quantification of the myotube diameter, as described in the Materials and Methods section. Data are expressed as the mean ± SD. ^aP < 0.05 vs. the control group; ^bP < 0.05 vs. the PA-treated group.