Research Paper Volume 11, Issue 8 pp 2217—2240

Figure 6. RSV activates the PKA/LKB1/AMPK signaling pathway. (A) Representative images of the western blotting results for p-LKB1, LKB1, p-AMPK and AMPK in myotubes; GAPDH was used as a loading control. (B) The bar charts show quantification of the indicated proteins. (C) Quantitative graph shows the ratios of p-AMPK/AMPK. (D) Representative images of the western blotting results for p-PKA, PKA, p-LKB1, LKB1, p-AMPK and AMPK in myotubes; GAPDH was used as a loading control. (E) The bar charts show quantification of the indicated proteins. (F) Quantitative graph shows the ratios of p-LKB1/LKB1 and p-AMPK/AMPK. Data are expressed as the mean ± SD. ^aP < 0.05 vs. the control group; ^bP < 0.05 vs. the PA-treated group; ^cP < 0.05 vs. the RSV (25 μM) and PA (0.75 mM) cotreated group with control siRNA transfection. (G) Representative images of the western blotting results for p-PKA, PKA, p-LKB1, LKB1, p-AMPK and AMPK in the GAS muscles; GAPDH was used as a loading control. (H) The bar charts show the relative protein levels. Data are expressed as the mean ± SD. ^aP < 0.05 vs. the O group; ^bP < 0.05 vs. the HO group.