Research Paper Volume 11, Issue 11 pp 3668—3678

Figure 3. Apigenin inhibits HSF cell survival by suppressing FAK phosphorylation. (A) HSFs overexpressing vector or FAK were treated with 20 μM apigenin, after which MTT assays were performed at the indicated times. Results depict the mean±SD of three experiments done in triplicate. **P < 0.05 vs. NaCl group; ##P < 0.05 vs. NaCl with FAK overexpression group. (B) HSFs transfected with si-FAK or si-NC (negative control) were treated with 20 μM apigenin, after which MTT assays was performed at the indicated times. Results depict the mean±SD of three experiments done in triplicate. **P < 0.05 vs. NaCl group. (C, D) HSFs overexpressing empty vector or FAK were treated with 20 μM apigenin, after which western blot and real-time PCR assays were performed to detect levels of cyclin D1 expression. **P < 0.05 vs. NaCl group; ## P < 0.05 vs. NaCl with FAK overexpression group. (E, F) HSFs transfected with si-FAK or si-NC (negative control) were treated with 20 μM apigenin, after which western blot and real-time PCR assays were performed to detect levels of Cyclin D1 expression. **P < 0.05 vs. NaCl group.