Research Paper Volume 11, Issue 17 pp 6657—6673

Figure 6. MIR4435-2HG regulates Wnt/β-catenin signaling through DSP to promote GC tumorigenesis and progression. (A) CCK8 assays were used with the HGC-27 cells co-transfected with shMIR4435-2HG and si-DSP (**P < 0.01). (B) The incidence of apoptosis among HGC-27 cells co-transfected with shMIR44352HG and si-DSP was determined using flow cytometry (**P < 0.01). (C, D) Transwell assays were used to assess cell migration and invasion in the group co-transfected with shMIR4435-2HG and si-DSP (**P < 0.01). (E) WB was performed to assess expression of β-catenin and lamin B1. (F) Levels of E-cadherin, N-cadherin, vimentin, c-Myc, cyclin D1, survivin and β-catenin within tumor tissue were determined using WB. (G) TCF/LEF transcriptional activity was determined by dual-luciferase assays in HGC-27 cells in response to MIR4435-2HG inhibition.