Research Paper Volume 11, Issue 19 pp 8484—8501

Figure 7. Validation of the transcript (mRNA) and protein levels of the hub genes using the Gene Expression Profiling Interactive Analysis (GEPIA) and The Human Protein Atlas database. (A) The transcript (mRNA) of the hub genes, namely, CXCR4, HIF1A, SDC1, TWIST1, and ZEB1 in PAAD and normal pancreatic tissues are shown. (B) Immunohistochemical (IHC) staining data of hub genes as follows: HIF1A protein expression in a PAAD tumor tissue (Staining: low; Intensity: weak; Quantity: 75%-25%; Location: cytoplasmic/membrane); HIF1A protein expression in a representative normal pancreatic tissue (Staining: low; Intensity: weak; Quantity: 75%-25%; Location: nuclear); SDC1 protein expression in a PAAD tumor tissue (Staining: medium; Intensity: moderate; Quantity: 75%-25%; Location: cytoplasmic/membrane). SDC1 protein expression in a representative normal pancreatic tissue (Staining: low; Intensity: moderate; Quantity: < 25%; Location: cytoplasmic/membrane). ZEB1 protein expression in a PAAD tumor tissue (Staining: low; Intensity: weak; Quantity: > 75%; Location: cytoplasmic/membrane). ZEB1 protein expression in a representative normal pancreatic tissue (Staining: low; Intensity: moderate; Quantity: < 25%; Location: cytoplasmic/membrane). The database lacked information regarding CXCR4 and TWIST1 protein expression in PAAD tumor tissues and normal pancreatic tissues. IHC results consistent with changed trend of transcript (mRNA) of hub genes in GSE60980 (GPL14550) and GEPIA were displayed.