Research Paper Volume 11, Issue 21 pp 9846—9861
Iron overload resulting from the chronic oral administration of ferric citrate induces parkinsonism phenotypes in middle-aged mice
Figure 4. Oxidative stress-induced neuronal loss is implicated in the neurotoxicity of ferric citrate supplementation. (A and B) Representative images of immunohistochemical 4-HNE staining show the accumulation of lipid peroxidation in the CPu and SN induced by ferric citrate supplementation. (C–E) Quantifications show the increased peroxidation of lipids, DNAs and proteins in the CPu and SN induced by ferric citrate supplementation. (F) Quantification shows the decreased GSH levels in the CPu and SN induced by ferric citrate supplementation. (G) qRT-PCR shows the decreased mRNA levels of typical antioxidant genes in the CPu and SN of mice supplemented with ferric citrate (N=5). (H) Quantification shows the decreased activities of SOD in the CPu and SN induced by ferric citrate supplementation. Error bars indicate SEM. Bars, 100 μm. Compared with the Ctr group, *p<0.05 and **p<0.01. Compared with the 1.25% ferric citrate group, #p<0.05 and ##p<0.01.