Research Paper Volume 12, Issue 1 pp 138—155
Lycorine induces autophagy-associated apoptosis by targeting MEK2 and enhances vemurafenib activity in colorectal cancer
Figure 2. Lycorine interacts with mitogen-activated protein kinase kinase 2 (MEK2) in a molecular docking model. (A) Lycorine directly bound to MEK2 via conventional hydrogen bonds at LYS101, ASP194, LYS196, and ASN199 in the docking structure. (B) Twenty conformations acquired from the flexible docking model between lycorine and MEK2. (C) The description and position of the interaction sites, including an ATP-binding region and a proton acceptor region. (D–E) Suppression of the phosphorylation of MEK2 and its downstream target ERK by lycorine in SW480 and HCT116 cells. Protein expression was analyzed using western blotting with the indicated antibodies. Data are presented as the mean ± SD of three independent experiments (****p < 0.0001).