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Research Paper Volume 12, Issue 1 pp 370—386
Long noncoding RNA VPS9D1-AS1 augments the malignant phenotype of non-small cell lung cancer by sponging microRNA-532-3p and thereby enhancing HMGA2 expression
Figure 5. VPS9D1-AS1 acts as a ceRNA of miR-532-3p and thereby upregulates HMGA2 expression. (A, B) HMGA2 mRNA and protein levels in H460 and A549 cells determined by RT-qPCR and western blot, respectively, after transfection with si-VPS9D1-AS1 or si-NC. *P < 0.05 vs. the si-NC group. (C) Efficiency of transfection of H460 and A549 cells with antagomir-532-3p determined by RT-qPCR. *P < 0.05 vs. the antagomir-NC group. (D–F) Expression levels of miR-532-3p, HMGA2 mRNA, and HMGA2 protein in H460 and A549 cells after co-transfection with si-VPS9D1-AS1 and antagomir-532-3p or antagomir-NC determined using RT-qPCR and western blot, respectively. *P < 0.05 vs. the si-NC group. #P < 0.05 vs. the si-VPS9D1-AS1 + antagomir-NC group.