Research Paper Volume 12, Issue 2 pp 1256—1271

Figure 1. Ubiquinol-10 rescued aging-induced fragmentation of porcine oocytes. (A) Timeline diagram of postovulatory oocytes aging. Oocyte morphologies (B) and fragmentation rate (C) in aging 0 h, 24 h, and 48 h groups. The red dotted circle indicates fragmented oocytes. Original magnification, 3.5×. Scale bars represent 100 μm. ***p < 0.001 indicate significant differences. (D) Oocyte morphologies in Ctrl-48 h and 100 μM Ubi-48 h groups. The red dotted circle indicates fragmented oocytes. Scale bars indicate 100 μm. (E) The fragmentation rate of aging 48 h oocytes treated with different concentrations of ubiquinol-10 (0, 50, 100, 200, or 400 μM). Different letters indicate significant differences, p < 0.05. Ctrl-48 h: oocytes aging 48 h without ubiquinol-10. Ubi-48 h: oocytes aging 48 h with ubiquinol-10.