Research Paper Volume 12, Issue 3 pp 2142—2155

Figure 5. Effect of BC on the BPH-related and apoptosis related protein and mRNA expression in RWPE-1 cells. (A) Effect of BC on the cell viability in RWPE-1 cells. RWPE-1 cells were treated with 3.13 to 200 μM of BC for 24 h. (B) Inhibitory effect of BC on cell proliferation in DHT-stimulated RWPE-1 cells. RWPE-1 cells were treated with 10 nM DHT, with or without 6.25–200 μM of BC for 24h. (C–E) RWPE-1 were stimulated with 10 nM DHT from 3days to 5days, with or without BC (25, 50 μM). (C) The mRNA level of AR, PSA, PCNA, Bax, Bcl-xL and Bcl-2 were quantificated using RT-PCR. RWPE-1 cell lysates were immunoblotted with (D) AR, PSA, PCNA (E) Bax, Bcl-2 primary antibodies. Protein levels which are normalized by internal control β-actin are represented as relative protein levels. P value ### = P < 0.001 versus vehicle group; * = P < 0.05, ** = P <0.01, *** = P < 0.001 versus the DHT-stimulated group.