Figure 4. Validation of miRNA expression by using qPCR. (A) 6yes9no group, (B) 6no9yes group, and (C) 6yes9yes group. The expression levels of miRNAs were quantified relative to U6 expression level by using the comparative cycle threshold (ΔCT) method. Data are presented as means ± SD (n = 3, *p < 0.05, **p < 0.01).