Research Paper Volume 12, Issue 6 pp 5091—5120

Figure 9. In vitro response to increasing concentrations of ADP of whole blood platelets from mice with orthotopic model of breast cancer. Results are presented as median (horizontal line) and interquartile range (box) (n = 8). The expressions of the active form of GPIIb/IIIa (A, B) and the binding of endogenous fibrinogen (Fg) (C, D) on platelets stimulated with 5 μM ADP (A, C) and 20 μM ADP (B, D) were measured using flow cytometry in non-fixed ‘washed blood’ withdrawn immediately (t₀) or after 2 (t₂), 3 (t₃), 4 (t₄) or 5 weeks (t₅) from the injection of 4T1 cells. Results are expressed as the percentage fraction of a given activation marker-positive platelets. More experimental details are given in the Materials and methods section. Statistical significance of differences, estimated with Kruskal-Wallis test followed by post hoc all-pairwise comparisons Conover-Inman or one-way ANOVA followed by Tukey’s multiple comparisons test, was: active form of GPIIb/IIIa_ADP5μM,; P_1,α < 0.05, 4T1 t₅ < 4T1 t₄ = 4T1 t₃ > 4T1 t₂ = 4T1 t₀; active form of GPIIb/IIIa_ADP20μM, P_1,α < 0.01, 4T1 t₄ > 4T1 t₂; Fg_ADP5μM, P_1,α < 0.05, 4T1 t₅ = 4T1 t₄ > 4T1 t₃ = 4T1 t₂ = 4T1 t₀; Fg_ADP20μM, P_1,α < 0.05, 4T1 t₅ = 4T1 t₄ > 4T1 t₃ < 4T1 t₂ > 4T1 t_0.