Research Paper Volume 12, Issue 9 pp 7660—7678
HOTAIR drives autophagy in midbrain dopaminergic neurons in the substantia nigra compacta in a mouse model of Parkinson’s disease by elevating NPTX2 via miR-221-3p binding
Figure 6. HOTAIR induces autophagy via miR-221-3p dependent NPTX2 elevation in PD mouse models. The retention time of PD mice in the rota rod test. (A) The number of TH positive cells in midbrain SNc tissues of PD mice tested by IHC. (B) The expression rate of NPTX2 in SNc tissues of PD mice detected by IHC. (C) The ratios of LC3B-I/LC3B-II and LAMP1/LAMP2 along with the P62 expression patterns in midbrain SNc tissues of PD mice measured by Western blot analysis (n = 10). (D) *p < 0.05 vs. PD mice injected with saline, #p < 0.05 vs. PD mice injected with MPTP + LV-oe-NC + agomir-NC, &p < 0.05 vs. PD mice injected with MPTP + LV-oe-HOTAIR + agomir-NC. Data (mean ± standard deviation) among multiple groups were analyzed using one-way ANOVA and subjected to Tukey’s post-hoc test. n = 10. PD, Parkinson’s disease; HOTAIR, HOX transcript antisense intergenic RNA; miR-221-3p, microRNA; NPTX2, neuronal pentraxin II; TH, T helper; SNc, substantia nigra compact; IHC, immunohistochemistry; MPTP, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyrindine; NC, negative control.