Research Paper Volume 12, Issue 9 pp 7717—7728

Figure 4. RES rescued DXR-induced apoptosis through DNA-damage-P63-Caspase3 pathway in mouse oocytes. (A) Representative immunofluorescence images showing the expression of γ-H2AX in mouse oocytes. Green, γ-H2AX, Blue, DNA, Bar = 20 μm. (B) The relative immunofluorescence intensity of γ-H2AX was measured in control, DXR-treated and RES-supplemented oocytes. Experiments were repeated at least 3 times with more than 30 oocytes examined for each group. Data were presented as means ± S.E.M of three independent experiments. **means P < 0.01, *** means P < 0.001. (C) Protein levels of γ-H2AX, P63 and Active-Caspase3 were examined by Western blotting in control, DXR-treated and RES-supplemented oocytes. GAPDH was used as a loading control. The clean backgrounds for the active-Caspase-3, γ-H2AX and GAPDH is due to the exposure.