Research Paper Volume 12, Issue 9 pp 8107—8119
Nur77 attenuates inflammatory responses and oxidative stress by inhibiting phosphorylated IκB-α in Parkinson’s disease cell model
Figure 6. Effects of Nur77 on cytokines and oxidant stress in primary neurons. MPP+ significant upregulated in the expression of mRNAs encoding (A–C) TNF-α, IL-6 and MCP-1 and downregulated (D–F) Nrf2, HO-1, NQO-1, while siIκB-α can reverse the upregulation and downregulation. The role of CSN-B is the same as siIκB-α, while siNur77 can significantly increase level of TNF-α, IL-6 and MCP-1 and decrease level of Nrf2, HO-1, NQO-1. When IκB-α is silenced by IκB-α siRNA, the regulative role of CSN-B and siNur77 is significantly reduced (***P < 0.001; compared with control group; $$$P < 0.001,$$ P < 0.01,$P < 0.05, compared with MMP+ group; #P<0.05 compared with SiNur77+MPP+ group; ΔP<0.05 compared with CSN-B+MPP+ group; n=3, mean +/- SEM).