Research Paper Volume 12, Issue 9 pp 8241—8260

Figure 2. LncRNA-MALAT1 transferred by exosomes to cardiomyocytes. (A) Heat map of the lncRNAs differentially expressed between exosomes derived from MSCs pretreated with hypoxia (exosome^Hypoxia) and exosomes derived from MSCs without any treatment (exosome). (B) Relative lncRNA-MALAT1 expression was validated by qRT-PCR in exosome^Hypoxia and exosome; ^*P < 0.05 versus exosome; (C) LncRNA-MALAT1 mRNA was examined by qRT-PCR in cardiomyocytes incubated with exosome^Hypoxia or exosome. The cardiomyocytes without any treatment were used as control; ^*P < 0.05 versus control; ^▲P < 0.05 versus exosome^Hypoxia. (D) The siRNA-mediated transfection efficiency in MSCs was demonstrated by qRT-PCR. ^*P < 0.05 versus siRNA-lncRNA-MALAT1. (E) LncRNA-MALAT1 mRNA in exosomes was examined by qRT-PCR. Each column represents the mean ± SD of three independent experiments. ^*P < 0.05 versus control; ^▲P < 0.05 versus hypoxia + siRNA-lncRNA-MALAT1. (F) LncRNA-MALAT1 mRNA in cardiomyocytes was examined by qRT-PCR. ^*P < 0.05 versus control; ^▲P < 0.05 versus exosome^Hypoxia; °P < 0.05 versus exosome^{Hypoxia+siRNA-LncRNA-NT}