Research Paper Volume 12, Issue 9 pp 8352—8371

Figure 4. HT29 cell-derived exosomes promote proliferation, migration and tube formation abilities of HMEC-1 cells by inhibiting FOXO1. (A) EdU assay was applied to detect proliferation of HMEC-1 cells after treatment of HT29-Exo and rAd-FOXO1 (Scale bar = 50 μm); (B) HMEC-1 cell migration was detected by Transwell assay after treatment of HT29-Exo and rAd-FOXO1 (Scale bar = 50 μm); (C) tube formation abilities of HMEC-1 cell were detected by tube formation assay after treatment of HT29-Exo and rAd-FOXO1 (Scale bar = 100 μm); (D–E) expression of angiogenesis-related proteins (VEGFA, VEGFAR2, ANG2, PIGF, MMP-2 and MMP-9) and FOXO1 in HMEC-1 cells after treatment of HT29-Exo and rAd-FOXO1 was detected by western blot analysis; *p < 0.05 compared with the PBS + GFP group, #p < 0.05 compared with the HT29-Exo + GFP group; Measurement data were presented as mean ± standard deviation; comparisons among multiple groups were assessed by one-way analysis of variance. Cell experiment was repeated three times.