Research Paper Volume 12, Issue 9 pp 8605—8621

Figure 2. MXRA5 is strongly upregulated in BPH tissues compared with the normal ones. (A) Upregulation of MXRA5 mRNA expression in BPH analyzed by Oncomine database. Analysis using the Oncomine database revealed increased MXRA5 at transcriptional level in BPH stromal tissues versus normal prostate stroma. (B) qRT-PCR analysis showed that the gene expression of MXRA5 in BPH tissues (n = 15) was significantly higher than the normal prostate tissues (n = 15). The GAPDH mRNA was used as an internal control, ** means P<0.01. (C) Western blot analysis revealed a strongly increasing protein abundance of MXRA5 in BPH tissues versus normal ones. (D) Relative densitometric quantification of MXRA5 protein in BPH tissues versus normal ones. GAPDH expression was analyzed as a loading control, results are expressed as ratio of the proteins in respect to GAPDH. ** means P < 0.01 vs. normal prostates.