Research Paper Volume 12, Issue 9 pp 8669—8679

Figure 6. Accelerated ossification in Col10a1-TAp63γ transgenic mice. (A) Col10a1 distal promoter and a shorter Col10a1 basal promoter (ShXBP) (from −220 to +45 bp) were used to generate a p63-expressing transgenic construct. (B) PCR genotyping was performed for the Col10a1-TAp63γ transgenic mice using DNA prepared from skin. (C) For mice at postnatal day 1 (P1), ossification signals of the fore- and hind-limb digits were evaluated. Tail ossification signals were observed up to the 11^th caudal vertebra in transgenic mice and up to the 8^th caudal vertebra in WT mice. (D) The statistical analyses of the ossified caudal vertebrae from four Col10a1-TAp63γ transgenic mouse lines at P1 are presented. Line 2: n = 9; Line 5: n = 9; Line 7: n = 10.