Research Paper Volume 12, Issue 11 pp 10594—10613

Figure 5. SOX2 transcriptionally activates F2R expression. (A) By resorting to the data collected from Sun glioblastoma dataset (GSE4290), we picked out top-ranked genes co-expressed with F2R. (B) The co-expression of SOX2 and F2R was validated using data from GSE4290. Correlation coefficient r=0.5066, P<0.01. (C) GSEA analysis based on the TCGA glioma database revealed that F2R was associated with SOX2 targets. (D, E) The mRNA expression level of SOX2 and F2R were detected in 16 paired glioma and adjacent normal tissues using RT-PCR. (F) The correlation of SOX2 and F2R was validated using RT-PCR. Correlation coefficient r=0.6895, P<0.01. (G, H) The protein and mRNA level of F2R were measured under the condition of SOX2 overexpression in U87 and U251 cell lines. (I) Transcription factor online prediction analysis revealed two SOX2 binding site in the promoter region of F2R. A random region without SOX2 binding sites acted as a negative control (Neg). (J, K) ChIP result using anti-IgG antibody and anti-SOX2 antibody. (L) Relative luciferase activity of the control or F2R overexpression glioma cells. All experiments were repeated at least three times. Data are presented as mean ± SEM. *P<0.05; **P<0.01.