Research Paper Volume 12, Issue 11 pp 11025—11041

Figure 5. LncRNA ADAMTS9-AS2 regulated chemoresistance of GC cells to cisplatin by modulating cell pyroptosis. CCK-8 assay was performed to determine cell proliferation abilities in (A) CR-GC cells and (C) ACR-GC cells. Trypan blue staining assay was conducted to measure cell viability in (B) CR-GC cells and (D) ACR-GC cells. (E, F) Colony formation assay was used to detect colony formation abilities of CR-SGC7901 cells and CR-BGC-823 cells. Cell viability in (G) CR-GC cells and (H) ACR-GC cells were detected by trypan blue staining assay. (I) FCM assay (Related to Supplementary Figure 1) were performed to determine cell apoptosis ratio in CR-GC cells. (“OE-A” represented “overexpressed LncRNA ADAMTS9-AS2”, “OE-miR” represented “overexpressed miR-223-3p”, “Mic” represented “miR-223-3p mimic”). Each experiment repeated at least 3 times. “NS” represented “no statistical significance”, *P < 0.05, **P < 0.01.