Research Paper Volume 12, Issue 14 pp 14157—14173
Circular RNA hsa_circ_0000073 contributes to osteosarcoma cell proliferation, migration, invasion and methotrexate resistance by sponging miR-145-5p and miR-151-3p and upregulating NRAS
Figure 2. Hsa_circ_0000073 induced proliferation, migration and invasion of OS cells in vitro. (A) Using hsa_circ_0000073 sequence information, shRNAs and hsa_circ_0000073 overexpression plasmids of were constructed. (B) The overexpression and knockdown effects in MG-63 and U2OS cells were analyzed by qRT-PCR. (C, D) Edu staining and CCK-8 assays were conducted to confirm the influences of hsa_circ_0000073 overexpression and knockdown on the proliferation of MG-63 and U2OS cells. Magnification, ×100; Scale bar=100 μm. (E, F) Transwell assays were adopted to assess the changes in migration and invasion abilities in hsa_circ_0000073-overexpressing and hsa_circ_0000073-silenced MG-63 and U2OS cells. Magnification, ×100; Scale bar=100 μm. *P<0.05.