Research Paper Volume 12, Issue 14 pp 14157—14173
Circular RNA hsa_circ_0000073 contributes to osteosarcoma cell proliferation, migration, invasion and methotrexate resistance by sponging miR-145-5p and miR-151-3p and upregulating NRAS
Figure 6. Hsa_circ_0000073 promoted proliferation, migration and invasion of OS by targeting miR-145-5p and miR-151-3p. (A) Putative miR-145-5p or miR-151-3p-binding sequence of NRAS. (B) Dual-luciferase reporter gene assays were applied to analyze the luciferase activity. (C) An AGO2 RIP assay was adopted to examine the correlations between hsa_circ_0000073 and miR-145-5p or miR-151-3p. (D–F) MG-63 and U2OS cells were cotransfected with hsa_circ_0000073 plasmid and miR-145-5p or miR-151-3p mimics. Cell proliferation was studied using CCK-8 assays and EdU staining. Magnification, ×100; Scale bar=100 μm. (G–H) Transwell assays were applied to analyze the changes in cell migration and invasion. Magnification, ×100; Scale bar=100 μm. *P<0.05.