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Research Paper Volume 12, Issue 14 pp 14365—14375

Circular RNA circVEGFC accelerates high glucose-induced vascular endothelial cells apoptosis through miR-338-3p/HIF-1α/VEGFA axis

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Figure 3. circVEGFC sponged miR-338-3p in HUVECs. (A) Schematic diagram showed the binding within miR-338-3p and circVEGFC. (B) Bioinformatics prediction (https://circinteractome.nia.nih.gov/) indicated the complementary binding sites with circVEGFC. (C) Luciferase reporter assay uncovered the compact binding within miR-338-3p and wild type of circVEGFC in the co-transfection. (D) RT-qPCR revealed miR-338-3p leve with the concentration rising. (E) RT-qPCR revealed the miR-338-3p level in the concentration gradient treated HUVECs. (F) RT-PCR showed the miR-338-3p level when circVEGFC was silenced. (G) RNA Fluorescence in situ hybridization (RNA-FISH) unveiled the subcellular location of miR-338-3p and circVEGFC in HUVECs. *P < 0.05, **P < 0.01.