Research Paper Volume 12, Issue 23 pp 23609—23618
Inhibition of Exo-miR-19a-3p derived from cardiomyocytes promotes angiogenesis and improves heart function in mice with myocardial infarction via targeting HIF-1α
Figure 2. Downregulation of miR-19a-3p promotes endothelial cells survival and proliferation. (A) qRT-PCR analysis of miR-19a-3p level was measured in endothelial cells. U6 was used as the control. (B) TUNEL staining of endothelial cells after treatment of exosomes derived from CM-culture medium. (C) Flow cytometry was performed to detect apoptosis of endothelial cells. (D) Western blot analysis of expression level of CD31. (E) Immunofluorescence staining of ki67 in endothelial cells. (F) EdU staining in endothelial cells to show the effect of miR-19a-3p on proliferation of endothelial cells. (G) Flow cytometry was performed to detect cell cycle of endothelial cells. Exosome was derived from CM-culture medium. AMO-19 was transfected to inhibit the level of miR-19a-3p. **P < 0.01. All experiments were performed more than 3 biological repeats. Scar bar = 50μm.