Research Paper Volume 13, Issue 5 pp 7660—7675
Long noncoding RNA NR2F1-AS1 promotes the malignancy of non-small cell lung cancer via sponging microRNA-493-5p and thereby increasing ITGB1 expression
NR2F1-AS1-mediated sponging of miR-493-5p increases ITGB1 expression in H460 and A549 cells. (A, B) The mRNA and protein levels of ITGB1 in H460 and A549 cells transfected with si-NR2F1-AS1 or si-NC were measured by RT–qPCR and western blotting, respectively. (C) ITGB1 mRNA expression in 73 pairs of NSCLC tissues and matched adjacent normal tissues was determined by RT–qPCR. (D) Expression correlation between NR2F1-AS1 and ITGB1 mRNA in the 73 NSCLC tissues was identified by Pearson's correlation coefficient (r = 0.5704, P < 0.0001). (E) MiR-493-5p inhibitor or NC inhibitor was transfected into H460 and A549 cells, and the efficiency of miR-493-5p silencing was verified by RT–qPCR. (F, G) MiR-493-5p inhibitor or NC inhibitor was cotransfected into H460 and A549 cells with si-NR2F1-AS1. Expression change in ITGB1 mRNA and protein levels was evaluated by RT–qPCR and western blotting, respectively. *P < 0.05 and **P < 0.01.