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Research Paper Volume 13, Issue 5 pp 7660—7675
Long noncoding RNA NR2F1-AS1 promotes the malignancy of non-small cell lung cancer via sponging microRNA-493-5p and thereby increasing ITGB1 expression
NR2F1-AS1 silencing impairs NSCLC tumor growth in vivo. (A) Growth curves of volumes in tumor xenografts were determined based on tumor volume measured every week for 5 weeks. (B) Tumor xenografts derived from sh-NR2F1-AS1 or sh-NC stably transfected H460 cells were collected and photographed. (C) The weights of tumor xenografts in sh-NR2F1-AS1 and sh-NC groups were measured at 5 weeks after cell tumor cell inoculation. (D, E) RT–qPCR was utilized to detect expression levels of NR2F1-AS1 and miR-493-5p in tumor xenografts derived from sh-NR2F1-AS1 or sh-NC stably transfected H460 cells. (F) Western blotting was employed to determine ITGB1 protein expression in tumor xenografts obtained from sh-NR2F1-AS1 and sh-NC groups. **P < 0.01.