Research Paper Volume 12, Issue 19 pp 19012—19021

Ox-LDL reduced the expression of KLF6 mediated by JNK in HUVECs. (A, B) Cells were treated with ox-LDL (50, 100 μg/mL) for 24 h. mRNA and protein of KLF6 were measured by real-time PCR and western blot analysis respectively. (C) Cells were treated with ox-LDL (50, 100 μg/mL) for 2 h. Phosphorylated JNK was measured by western blot analysis. (D, E) Cells were treated with ox-LDL (100 μg/mL) in the presence of the JNK inhibitor SP600125 (10 μM) for 24 h. mRNA and protein of KLF6 were measured, respectively (**, ***, ****, P<0.01, 0.001, 0.0001 vs. non-treatment group;, ###, P<0.0001 vs. ox-LDL treatment group, n=4-5).