Research Paper Volume 12, Issue 19 pp 19095—19106

Ovarian cancer cells deliver circ-0001068 into T cells to induce PD1 expression by sponging miR-28-5p through exosomes. (A) The relative levels of circ-0001068 in the exosomes from the ovarian surface epithelium (OSE) cell line, (IOSE80) and ovarian cancer cell lines (HOSE, CAOV3, A2780, and HO-8910). (B) The relative levels of circ-0001068 in the Jurkat T cells that were incubated with exosomes from HOSE cells, A2780 cells, and A2780 cells that were transfected with circ-0001068 siRNA. (C) The relative levels of miR-28-5p in the Jurkat T cells that were incubated with exosomes from HOSE cells, A2780 cells, A2780 cells that were transfected with circ-0001068 siRNA, and A2780 cells plus an miR-28-5p mimic. (D) Flow cytometry was used to determine the relative levels of PD1 in the Jurkat T cells that were incubated with exosomes from HOSE cells, A2780 cells, A2780 cells that were transfected with circ-0001068 siRNA, and A2780 cells plus an miR-28-5p mimic. Right: representative image; Left: quantitative analysis. (E) The relative levels of circ-0001068 in the Jurkat T cells that were incubated with serum exosomes from healthy volunteers, ovarian cancer patients, and ovarian cancer patients plus an miR-28-5p mimic. (F) The relative levels of miR-28-5p in the Jurkat T cells that were incubated with serum exosomes from healthy volunteers, ovarian cancer patients, and ovarian cancer patients plus an miR-28-5p mimic. (G) The relative levels of PD1 in the Jurkat T cells that were incubated with serum exosomes from healthy volunteers, ovarian cancer patients, and ovarian cancer patients plus an miR-28-5p mimic. Right: representative image; Left: quantitative analysis. ***P < 0.001.