Research Paper Volume 12, Issue 19 pp 19147—19158
BRD4 inhibition sensitizes renal cell carcinoma cells to the PI3K/mTOR dual inhibitor VS-5584
VS-5584 induces apoptosis activation in RCC 786-O cells. RCC 786-O cells were treated with applied concentrations of VS-5584 (0.5-10 μM), cells were further cultured for the indicated time; Cell death was tested by LDH medium release assay (A); Cell apoptosis was tested by ssDNA ELISA (B), Western blotting testing apoptosis proteins (C), and nuclei TUNEL staining (D). Data were presented as mean ± standard deviation (SD, n=5). *p< 0.05 vs. “C” group. The in vitro experiments were repeated four times, and similar results were obtained.