Research Paper Volume 12, Issue 19 pp 19273—19292

Relative USP18 expression in pancreatic cancer and its clinical significance. (A, B) Determination (A) and quantification (B) of USP18 mRNA levels in pancreatic cancer tissues and in paired normal tissues by qRT-PCR analysis. Tubulin was used as a loading control. ^**p<0.01. (C and D) Relative expression of USP18 in pancreatic cancer tissues and adjacent non-tumour tissues by western blotting analysis. Statistical differences were analysed using the paired t test. ^**p<0.01. (E and F) Representative images (E) and quantification (F) of USP18 staining in paired pancreatic cancer tissues. Scale bar, 50 μm. (G and H) Relationship between USP18 expression with poor overall survival and disease-free survival in pancreatic cancer patients.