Research Paper Volume 12, Issue 19 pp 19273—19292
Deubiquitinase USP18 promotes the progression of pancreatic cancer via enhancing the Notch1-c-Myc axis
USP18 promotes pancreatic cancer cell growth by upregulating c-Myc expression. (A, B) analyses were performed to detect the USP18 and C-Myc expression levels in BxPC-3 cells transfected with shUSP18 or shNC. (C and D) Western blot and qRT-PCR analyses were performed to detect the USP18 and C-Myc expression levels in SW1990 cells transfected with p-USP18 or vector. (E–G) Determination and quantification of c-Myc protein levels in pancreatic cancer tissues and paired non-tumour tissues by western blotting. Tubulin served as a loading control. (H) Scatter plots of USP18 and c-Myc protein expression in pancreatic cancer. (I) Determination of c-Myc mRNA levels in pancreatic cancer tissues and paired non-tumour tissues by qRT-PCR. (J) Scatter plots of USP18 and c-Myc mRNA expression in pancreatic cancer.