Research Paper Volume 12, Issue 19 pp 19293—19315

Up-regulated myeloid cell leukemia (Mcl)-1L and down-regulated Mcl-1S expression is correlated with tumor proliferation and poor survival in human gastric cancer (GC). (A) Comparison of Mcl-1L and Mcl-1S messenger RNA (mRNA) expression between GC and normal tissues was performed. Dot plots represent Mcl-1L and Mcl-1S mRNA expression levels and Mcl-1S/Mcl-1L ratios in 59 GC samples and 31 healthy tissues. Mcl-1L and Mcl-1S expression was normalized to glyceraldehyde 3-phosphate dehydrogenase expression. Data are presented after logarithmic transformation as the mean ± standard deviation (SD). *p <0.05, **p <0.01, ***p <0.001, versus the control. (B) The Mcl-1S/Mcl-1L mRNA ratios in cells in different T or N stages of the TNM staging system are presented. Data are shown after logarithmic transformation. (C) Kaplan–Meier survival curves for 59 individuals grouped based on the median value of Mcl-1S/Mcl-1L are shown. (D) Increased Mcl-1L and decreased Mcl-1S mRNA expression levels and Mcl-1S/Mcl-1L values in GC cell lines, compared with in the GES-1 cell line, are shown. mRNA expression was normalized by the 2^-ΔΔCt method. Data are shown as the means ± SD. (E) Western blot showing decreased Mcl-1S protein levels and Mcl-1S/Mcl-1L in GC tissues and cell lines is shown. (F) Increased Mcl-1L and decreased Mcl-1S protein expression levels and Mcl-1S/Mcl-1L values in GC cell lines, compared with those in the GES-1 cell line, are shown. Data are shown as the means ± SD.