Research Paper Volume 12, Issue 19 pp 19293—19315
Modification of Mcl-1 alternative splicing induces apoptosis and suppresses tumor proliferation in gastric cancer
The myeloid cell leukemia (Mcl)-1 splicing shifts from Mcl-1L to Mcl-1S after injection of the vivo-morpholino-modified steric-blocking oligonucleotides (SBOs) in the mouse xenograft models of MKN-45 and HGC-27 cells. (A) Mcl-1L and Mcl-1S messenger RNA (mRNA) expression in the MKN-45 and HGC-27 xenograft models treated with vivo-morpholino-modified SBOs at different dosages is presented. Data are shown as the means ± standard deviation (SD). **p <0.01, ***p <0.001, versus the control. (B) Mcl-1L and Mcl-1S protein expression in the xenograft models after treatment with SBOs at indicated doses is shown. (C) Decreased Mcl-1L and increased Mcl-1S protein expression levels and Mcl-1S/Mcl-1L values after SBO treatment are presented. Data are shown as the means ± SD. *p <0.05, **p <0.01, ***p <0.001 versus the control.