Research Paper Volume 12, Issue 19 pp 19335—19351

Correlation of FREM1 expression with immune infiltration level in the subtypes of breast cancer. (A) FREM1 expression is negatively related to tumor purity and has significant positive correlations with infiltrating levels of B cells, CD8+ T cells, CD4+ T cells, macrophages, neutrophils and dendritic cells in BRCA. (B) FREM1 expression is negatively related to tumor purity and has positive correlations with infiltrating levels of B cells and CD8+ T cells, but not CD4+ T cells, macrophages, neutrophils and dendritic cells in BRCA-Basal. (C) FREM1 expression is significantly negatively related to tumor purity and has significant positive correlations with infiltrating levels of CD8+ T cells, CD4+ T cells and dendritic cells, but not B cells, macrophages and neutrophils in BRCA-HER2. (D) FREM1 expression is negatively related to tumor purity and has significant positive correlations with infiltrating levels of CD8+ T cells, CD4+ T cells, macrophages, neutrophils and dendritic cells, but not B cells in BRCA-Luminal. (E) The top 50 highest TILRR-expressing and top 50 lowest TILRR-expressing tumor tissue samples were selected (TCGA database) to analyze the expression of marker genes in CD8+, CD4+ and T cells (general). (F) CD8A, CD8B and CD4 expression levels were analyzed by Q-PCR in the 5 highest TILRR expressing and the 5 lowest TILRR expressions samples.