Research Paper Volume 12, Issue 19 pp 19365—19374
Knockdown of NHP2 inhibits hepatitis B virus X protein-induced hepatocarcinogenesis via repressing TERT expression and disrupting the stability of telomerase complex
Overexpression of HBx in PLC/PRF5 hepatoma cells increases TERT and NHP2 expression. (A) The HBx mRNA expression was determined after PLC/PRF5 cells were transfected with ov-NC, ov-HBx-1 or ov-HBx-2 plasmids. (B) The protein expression of HBx, TERT and NHP2 was measured in the absence or presence of HBx stable expression. Ov: overexpression, NC: negative control, HBx: Hepatitis B virus X, TERT: telomerase reverse transcriptase. ***P<0.001.