Research Paper Volume 12, Issue 19 pp 19375—19398

Figure 3. HOXC10 promotes OC cell migration by regulating Slug transcription. (A) The GSEA plot indicates that HOXC10 expression is positively correlated with TGF-β and FAK pathway signatures (OC sample data were downloaded from TCGA, n=379). (B, C) mRNA expression of EMT-related genes in 8910 cells transfected with HOXC10 siRNA, negative control siRNA, the HOXC10 overexpression plasmid, and empty vector. P=0.0257 and P=0.0093. (D) 8910 cells were cotransfected with a plasmid containing the full-length Slug promoter and increasing concentrations of the HOXC10 overexpression plasmid. P=0.0499, P=0.0032 and P=0.0079. (E) 8910 cells were cotransfected with the plasmid containing the full-length Slug promoter and increasing concentrations of the HOXC10 NLS mutation plasmid. P=0.1005, P=0.9559 and P=0.4059. (F) Schematic diagram of three predicted HOXC10 binding sites in the Slug promoter region. (G) Relative fold enrichment for IgG at the three predicted binding sites, as evaluated by ChIP-qPCR. P=0.0162, P=0.0158 and P=0.0003. (H) Relative fold enrichment for IgG at the three predicted binding sites after deletion of the HOXC10 DNA binding sites. P=0.5259, P=0.3579 and P=0.8293. (I, J) Relative mRNA and protein expression levels of Slug in 8910 cells transfected with the Slug overexpression plasmid and empty vector. P=0.0018, P=0.0044. (K–L) Transfection efficiencies of the Slug siRNA products. P=0.0048, P=0.0001, and P=0.0013; P=0.0083, P<0.0001, and P=0.0002.