Priority Research Paper Volume 12, Issue 19 pp 18804—18821
The slc4a2b gene is required for hair cell development in zebrafish
slc4a2b knockdown leads to decreased HCs in the posterior lateral line neuromast of zebrafish. (A, B) slc4a2b-morphants (MO) (B) had fewer HCs in each neuromast compared to controls (A). Tg(Brn3c:mGFP) transgenic zebrafish were used in the analysis, and the cells labeled by GFP represent HCs, while DAPI was used to stain the cell nuclei. (C, D) Quantification of the number of HCs in each neuromast and the total HCs in posterior lateral line neuromasts of the slc4a2b-morphants and controls. Scale bar = 20 μm, *** P < 0.001).