Priority Research Paper Volume 12, Issue 19 pp 18804—18821
The slc4a2b gene is required for hair cell development in zebrafish
slc4a2b mRNA injection can rescue the phenotype induced by slc4a2b gene deficiency. The Tg(Brn3c:mGFP);slc4a2bmut zebrafish that were injected with Cas9 mRNA and slc4a2b sgRNA had fewer HC clusters (A, B) and fewer HCs in each posterior lateral line neuromast (A, C) compared to controls. However, slc4a2b mRNA injection could rescue the phenotype caused by slc4a2b mutation. *** P < 0.001; ** P < 0.01; ns, no significance.