Research Paper Volume 12, Issue 19 pp 19421—19439

Figure 2. Traumatic brain injury led to synaptic dysfunction. (A–C) Hippocampal CA3-CA1 LTP and its quantification (A) were recorded by using the MED64 system. Normalized CA3-CA1 fEPSP mean slope recorded from the CA1 dendritic region in hippocampal slices (B, C). (D, E) Representative dendritic spines of neurons from Golgi impregnated hippocampus (D) and averaged spine density (E) (mean spine number per 10 μm dendrite segment) were measured. Scale bar in left and right lower bar = 200 μm, bar in the middle panel = 2 μm. (F, G) Brain tissues from hippocampus were homogenized, and synaptic protein levels were detected by immunoblotting. n=3. p value significance is calculated from a one-way ANOVA, data are represented as mean ± SEM, *p < 0.05, **p < 0.01 vs control group.