Research Paper Volume 12, Issue 19 pp 19421—19439

Figure 6. Blockage of AEP abrogated the cytoplasmic retention of SET and attenuated tau pathology in TBI rats. (A, B) aAEP (molecular weight at 36KD) was significantly inhibited in TBI+AENK group, compared with the TBI group as evaluated by Western blotting. (C, D) The level of SET was significantly reduced in cytosolic fraction, but was increased in the nucleus (E, F) in TBI+AENK group compared with TBI group. (G) Brain hippocampal neurons PP2A level and activity were tested. (H–J) Western blotting (H) show significantly reduced levels of AT8 (I), pS199 (J) in the TBI+AENK group compared with the TBI group. (K, L) Normalized CA3-CA1 fEPSP mean slope recorded from the CA1 dendritic region in hippocampal slices. (M, N) Representative dendritic spines of neurons from Golgi impregnated hippocampus (M). Average spine density (N) (mean spine number per 10 μm dendrite segment) were measured. Scale bar = 2 μm. (O–Q) Hippocampal tissues were homogenized, and synaptic protein levels were detected by immunoblotting (O). Pre-synaptic proteins synaptophysin (P) and postsynaptic proteins PSD95 (Q). n=3. p value significance is calculated from a one-way ANOVA or two-way ANOVA, all data represent mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 vs TBI group.