Microglia in the auditory brain regions of NIHL mice assumed an activated morphology. (A–T) Representative z-projection images of Iba1-, CD68-, and DAPI-labeled control brain sections from the VCN (A–E), DCN (F–J), IC (K–O), and AC (P–T). (A’–T’) Representative images of corresponding brain sections from NIHL mice. The insets show higher magnifications of the corresponding Iba1+ cells signified by arrowheads. The scale bar equals 100 μm.