Research Paper Volume 12, Issue 19 pp 19660—19676
Anticancer effects of miR-124 delivered by BM-MSC derived exosomes on cell proliferation, epithelial mesenchymal transition, and chemotherapy sensitivity of pancreatic cancer cells
Exosomes-delivered miR-124 enhanced the chemotherapy on pancreatic cancers. The effects of BM-MSC-derived exosomes on AsPC-1 or PANC1 cell viability (A) were measured by MTT and apoptosis were determined using AnnexinV-propidium iodide (PI) by flow cytometry (B), while the apoptosis related protein (C) levels of caspase-3, Bax, Bcl-2 and EZH2 and EMT related proteins (D) N1CD, Hes1, MMP-9, vimentin and E-cadherin were determined by western blot. These results present the mean ± standard deviation (SD) of three independent experiments. ***P<0.001.