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Research Paper Volume 12, Issue 21 pp 21837—21853

Galectin-1 promotes vasculogenic mimicry in gastric adenocarcinoma via the Hedgehog/GLI signaling pathway

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Figure 4. LGALS1 promotes GLI1 expression in vitro. (A and C) Western blotting indicating that OE- LGALS1 efficiently induced GLI1 protein expression compared with wild-type control and negative control-transduced cells. (B and D) Transfection with shLGALS1#3 inhibited GLI1 protein expression compared with wild-type control and negative control-transduced cells. These results were confirmed at the mRNA level by qRT-PCR (E–H). GAPDH served as a loading control. (All n = 3, *P < 0.05, **P < 0.01).