Research Paper Volume 12, Issue 19 pp 19677—19700

Tissue specific regulation of the Akt and mTOR pathways in response to reduced mitochondrial protein synthesis. Akt and mTOR signaling were measured by immunoblotting of liver, heart and skeletal muscle lysates isolated from normal chow fed (A) and high fat diet (B) Ptcd1^+/+ (n=8) and Ptcd1^+/- (n=8) mice. GAPDH was used as a loading control. Relative protein levels and relative intensity of phosphorylation versus total protein levels were measured using ImageJ software and normalized to GAPDH. The data are representative of results obtained from 8 mice from each genotype. Error bars are SEM. *P < 0.05, Student’s t test. Terminal insulin testing of insulin dependent Akt activation was assessed in liver (C) and skeletal muscle (D) lysates isolated from normal chow fed and high fat diet Ptcd1^+/+ (n=6) and Ptcd1^+/- (n=6) mice. Relative protein levels and relative intensity of phosphorylation versus total protein levels were measured using ImageJ software and normalized to GAPDH. Error bars are SEM. *P < 0.05, Student’s t test.