Reduced mitochondrial translation prevents diet-induced metabolic dysfunction but not inflammation

Kara L. Perks; Nicola Ferreira; Judith A. Ermer; Danielle L. Rudler; Tara R. Richman; Giulia Rossetti; Vance B. Matthews; Natalie C. Ward; Oliver Rackham; Aleksandra Filipovska

doi:doi:10.18632/aging.104010

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Research Paper Volume 12, Issue 19 pp 19677—19700

Reduced mitochondrial translation prevents diet-induced metabolic dysfunction but not inflammation

HFD can recover mitochondrial biogenesis. Mitochondrial proteins (50 μg) isolated from Ptcd1^+/+ and two Ptcd1^+/- mice were resolved on 4-12% Bis-Tris gels and immunoblotted against antibodies to analyze the levels of nuclear and mitochondrial encoded proteins in liver (A, B) and skeletal muscle (C, D) isolated from normal chow diet (A, C) and high fat diet (B, D) fed Ptcd1^+/+ (n=6) and Ptcd1^+/- (n=6) mice. VDAC1 (porin) was used as a loading control. Relative abundance of proteins was measured using ImageJ software normalized to VDAC1. Error bars indicated SEM. *P < 0.05, Student’s t test.