Research Paper Volume 12, Issue 22 pp 22975—23003

Figure 4. KIF14 silencing reverses acquired resistance to sorafenib by downregulating p-AKT. (A) The protein expression profiles of sorafenib-resistant Huh7-SR and HepG2-SR cells and the corresponding parent cells were detected by western blotting. Representative protein bands are shown. The density of each band was normalized to that of β-actin. (B) Huh7-SR and HepG2-SR cells were transfected with control or siKIF14 for 48 h. The protein expression profiles were detected by western blotting. The density of each band was normalized to that of β-actin. (C–E) Huh7-SR and HepG2-SR cells were pretreated with 0 or 10 μM MK-2206 for 24 h, transfected with control or siKIF14, and incubated in a medium containing MK-2206 for another 24 h. Subsequently, the cells were harvested for analysis. The protein expression profiles were detected by western blotting. The density of each band was normalized to that of β-actin (C). The cell viability (%) of treated cells was compared with that of the corresponding untreated cells (D). The percentages of apoptotic cells (%) were measured by flow cytometry (E). Data represent three independent experiments. NS, not significant. “*” Indicates P<0.05 and “**” indicates P<0.001.