Research Paper Volume 12, Issue 19 pp 19740—19755
Development of a five-protein signature for predicting the prognosis of head and neck squamous cell carcinoma
Knockdown of HER3 suppresses the proliferation and invasion of HNSCC cells. (A) The expression level of HER3 mRNA is significantly lower in siHER3 treated cells compared to the siCTRL treated cells. (B) Western blot shows that the levels of HER3 and HER3_pY1289 are lower in siHER3 treated cells compared to the siCTRL treated cells. (C) MTT assay reveals that the OD values are lower in the cells subjected to HER3 downregulation. (D, E) The EdU assay shows that the percentage of EdU positive cells is lower in siHER3 treated cells compared to the siCTRL treated cells (scale bar=20 μm). (F, G) The invasion assay shows the number of cells invading the membrane is lower in siHER3 treated group than in the control group (scale bar=100 μm).