Research Paper Volume 12, Issue 23 pp 23836—23848

Figure 2. Hsa_circ_0001869 expression increased in NSCLC cell lines and knockdown of hsa_circ_0001869 expression suppressed cell proliferation. (A) qRT-PCR for hsa_circ_0001869 expression in A549, PC9, H1299, H1975, and H1650 tumor cells and the normal lung epithelial cell line HEAS-2B. n = 3. Data are expressed as mean ± SD. ^***P < 0.001 vs. normal group. (B) qRT-PCR analysis of expression of hsa_circ_0001869 in A549 and PC9 cells after knockdown of hsa_circ_0001869 (sh-circRNA). n = 3. Data are expressed as mean ± SD. ^***P < 0.001 vs. NC. (C) Cell cycle distribution by flow cytometry after PI staining. (D and E) CCK8 detection for the proliferation of A549 (D) and PC9 (E) cells with or without hsa_circ_0001869 silencing. n = 3. Data are expressed as mean ± SD. ^***P < 0.001 vs. NC. (F–H) Colony formation assay revealed colony-forming ability of A549 and PC9 cells. n = 3. Data are expressed as mean ± SD. ^***P < 0.001 vs. NC.